DiagPoly™ PEI PLGA Nanoparticles for Gene Delivery and Gene Therapy

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DiagPoly™ PEI PLGA Nanoparticles for Gene Delivery and Gene Therapy

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Creative Diagnostics provides biodegradable poly (d,l-lactide-co-glycolide) (PLGA) nanoparticles bearing polyethyleneimine (PEI) on their surface, DiagPoly™ PEI PLGA Nanoparticles, which is based on poly(lactide-co-glycolide) (PLGA) acid terminated with a lactide/glycolide ratio of 50/50 and a molecular weight of 32000 Da. These nanoparticles are positively charged due to the surface modification with PEI. PLGA-PEI nanoparticles are recommended for gene delivery and gene therapy.

Features:

  • Available in different sizes: 100 nm or 200 nm
  • Amphiphilic: both hydrophilic and lipophilic compounds can be encapsulated
  • Optional fluorescence: non- fluorescent, green fluorescent (EX/EM 498 nm/517 nm), red fluorescent (EX/EM 525 nm/548 nm)
  • Biodegradability, biocompatibility and controlled release
  • High efficiency for gene transfection and gene delivery
Cat No.DescriptionParticle SizeUnit SizeAdd to basket
DNQ-NV01PEI PLGA Nanoparticles0.1 µm50 mg
DNQ-NV02PEI PLGA Nanoparticles0.2 µm50 mg
DNQ-NV09PEI PLGA Nanoparticles, Fluorescent Green, L/G=50/500.1 µm50 mg
DNQ-NV10PEI PLGA Nanoparticles, Fluorescent Green, L/G=50/500.2 µm50 mg
DNQ-NV11PEI PLGA Nanoparticles, Fluorescent Red, L/G=50/500.1 µm50 mg
DNQ-NV12PEI PLGA Nanoparticles, Fluorescent Red, L/G=50/500.2 µm50 mg

* Other PEI PLGA Nanoparticles with different MW of PLGA can be customized upon request.

More and more attention has been paid on non-viral gene delivery vectors in recent years, such as liposomes (lipoplexes), polycationic polymers (polyplexes), and organic or inorganic nanoparticles (nanoplexes). To enhance gene delivery effect, various cationic complexes have been developed for delivering plasmid DNA, antisense, or siRNA into cells. Poly(d,l-lactide-co-glycolide) (PLGA) were extensively assessed for their ability to deliver a variety of therapeutic agents. PLGA nanoparticles were shown to escape from the endo-lysosomal compartment to the cytoplasmic compartment and release their contents over extended periods of time. These features rendered PLGA nanoparticles as potential tools for gene delivery efficiently.

Polyethylenimine (PEI), a cationic, water-soluble, linear, or branched polymer. Due to their high cationic charge density at physiological pH, PEIs are able to form non-covalent complexes with DNA, siRNA, and antisense oligodeoxynucleotide, which can be adsorbed onto negatively charged cell surface and translocated into cells via endocytic pathways. This DNA/PEI complexation can be achieved only at higher ratios of PEI to DNA, resulting in a strong positive surface charge. The intracellular release of PEI/nucleic acid complexes from endosomes is considered as relying on the protonation of amines in the PEI molecule, which leads to osmotic swelling and subsequent burst of the endosomes. Moreover, PEIs also facilitate nucleic acid entry into the nucleus. PEIs have been shown to be efficacious vectors in gene delivery. Their high transfection efficiency is believed to originate from the so-called “proton sponge effect”. Based on their high surface charge, PEIs are promising candidates as non-viral vectors for delivery of negatively charged nucleic acids, for in-vitro and in-vivo applications. Polyethylenimine (PEI) can be synthesized in different molecular weights and configurations, including linear or branched structures. Different PEIs have varying degrees of toxic effects on cells. In general, the transfection capability and cytotoxicity increases with the increase of molecular weight. Linear PEI shows lower cytotoxicity and higher efficiency than branched PEI for gene transfection. The transfection capability and cytotoxicity effect depend on the molecular weight and structure of PEIs. PEI coating may enhance the permeability of PLGA which has opposite charge.

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