Creative Diagnostics is providing a series of novel protein-based fluorescent nanoparticles which are very suitable for super-resolution cell imaging. The protein-based fluorescent nanoparticles are prepared with protein and fluorescent dye molecule by a crosslinker of EDC under benign ambient conditions. The protein molecule binds to the fluorescent dye molecule through the amino group of the surface, following aggregates the protein molecule by some clever methods, and then combine the labeled protein molecules with each other through the EDC cross-linking agent to form a protein-based fluorescent nanoparticles. The surface of the nanoparticles produced in this way leaves a large number of carboxyl groups outside which can be used to bind other biomolecules.

The protein-based fluorescent nanoparticles have some advantages of small size, high stability, long storage life and biocompatible compared to traditional agents, while retaining their biology activity. Furthermore, due to biocompatibility, the nanoparticles can enter the cells through internalization rapidly, thus the fluorescent dye molecules of the particles can be detected by fluorescence microscopy to achieve better cell imaging effects. During this process, glucose oxidase and glucose are necessary for producing hydrogen peroxide which can penetrate the cell membrane to allow the nanoparticles to enter the cell.

Features:

• Protein as material - Biocompatible, low toxicity, completely biodegradable
• Internalized quickly- Ideal for cell imaging
• Different Excitation/Emission wavelengths are available
• Tested Cell Lines: HeLa, A549, HN12 (others not tested)

Cat.No	Description	Surface	Diameter	Unit Size	Add to basket
PBF-G1001	Protein-Based Nanoparticles, Fluorescent, Excitation 350 nm	Carboxyl	10±2 nm	3 mg
PBF-G1002	Protein-Based Nanoparticles, Fluorescent, Excitation 425 nm	Carboxyl	10±2 nm	3 mg
PBF-G1003	Protein-Based Nanoparticles, Fluorescent, Excitation 494 nm	Carboxyl	10±2 nm	3 mg
PBF-G1004	Protein-Based Nanoparticles, Fluorescent, Excitation 580 nm	Carboxyl	10±2 nm	3 mg
PBF-G1005	Protein-Based Nanoparticles	Carboxyl	10±2 nm	3 mg
PBF-G1006	Protein-Based Nanoparticles, Fluorescent, Excitation 365 nm	Carboxyl	10±2 nm	3 mg
PBF-G4001	Protein-Based Nanoparticles, Fluorescent, Excitation 254 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4002	Protein-Based Nanoparticles, Fluorescent, Excitation 340 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4003	Protein-Based Nanoparticles, Fluorescent, Excitation 350 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4004	Protein-Based Nanoparticles, Fluorescent, Excitation 365 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4005	Protein-Based Nanoparticles, Fluorescent, Excitation 402 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4006	Protein-Based Nanoparticles, Fluorescent, Excitation 432 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4007	Protein-Based Nanoparticles, Fluorescent, Excitation 494 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4008	Protein-Based Nanoparticles, Fluorescent, Excitation 543 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4009	Protein-Based Nanoparticles, Fluorescent, Excitation 576 nm	Acid, base, hydroxyl, and others	35±5 nm	3 mg
PBF-G4010	Protein-Based Nanoparticles	Acid, base, hydroxyl, and others	35±5 nm	3 mg