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Home / Nanoparticles / Polymer Particles / Polystyrene (PS) / DiagPoly™ Ultra Multiple Fluorescent Polystyrene Particles, Flow Cytometry Grade, 3.5-3.9 µm

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DiagPoly™ Ultra Multiple Fluorescent Polystyrene Particles, Flow Cytometry Grade, 3.5-3.9 µm(DNS-SPR20U)

Datasheet Immobilization of Antibody on Beads

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DiagPoly™ Ultra Multiple Fluorescent Polystyrene Particles, Flow Cytometry Grade, 3.5-3.9 µm (# DNS-SPR20U) contains a single population of polystyrene particles with an enhanced UV and Far Red fluorescence intensity. Every particle contains a mixture of fluorophores that enable the particles to be excited at any wavelength from 365 nm to 650 nm. Using these particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Application Notes

Flow cytometry routine alignment.

Diameter

3.5-3.9 µm

Concentration

1.0E+7 particles/mL

Storage Buffer

DI water with 0.02% NaN3 and 0.01% NP40.

Unit Size

2 mL

Usage Statement

Routine calibration of flow cytometers. Dituiton of 1-2 drops to 1 mL will provide adequate number of particles for flow cytometry. Shake vigorously or vortex before use. Inclusion of a small amount of detergent in the diluent will help to increase the number of singles.

Storage

Room temperature, store at 4°C after first use for one year. Do not freeze. Protect from light.

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