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DiagNano™ Carboxyl Gold Nanoshells Conjugation Kits(GCK-150)

Datasheet

Kit for covalent conjugation of proteins and other amine containing ligands to 150 nm gold nanoshells (silica core). Contains enough reagents to run five experiments: 1 purification experiment, 3 conjugation experiments, ans optional 1 blocking experiment.

Kit Components
• Centrifugal purification column
• Centrifugal column tube
• 10 mL of purification buffer, 10 mM potassium phosphate
• 15 mL of 20 OD carboxyl gold nanoshells, 150 nm
• 50 mL of potassium phosphate reaction buffer, pH 7.4
• 50 mL of sodium phosphate reaction buffer, pH 7.4
• 1 mL of PBS reaction buffer, 50×PBS, pH 7.4
• PETG reagent bottle, empty (for dilution of 50×PBS reaction buffer)
• 10 mg of EDC, 3 each
• 10 mg of Sulfo-NHS, 3 each
• 100 µL of hydroxylamine quencher
• 5 mL of NCX block buffer
• 10 mL of NCX conjugate diluent
• 1.5 mL microcentrifuge tubes, 20 each
• 10 mL of NCX lateral flow running buffer
Diameter
150 nm
Functional Group
Carboxyl
Storage Buffer
0.02 mM potassium carbonate solution.
Unit Size
1 Kit
Absorption Max
780-830 nm
Storage
Storage: 2-8 ⁰C. DO NOT FREEZE.

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