DiagNano™ Silver Nanoparticle Covalent Conjugation Kit, 50 nm

(SCK-50)

Kit for one-step Medium Covalent conjugation of proteins and other amine containing ligands to 50 nm silver nanoparticles. Contains enough reagents for conjugation. 20 ug of protein can be conjugated in each reaction.

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Datasheet

Overview


Diameter
50 nm

Size Distribution
CV<12%

Absorption Max
425 nm

Kit Components
50nm NHS-Activated Silver Nanoparticles (lyophilized), Protein Resuspension Buffer, Reaction Buffer, Quencher Solution.

Functional Group
NHS (PEG5000-spacer between silver surface and NHS)

Application Notes
Ideal for development of peptide and protein silver conjugates for use in applications such as blotting, lateral flow assays, LSPR assays, SERS, light microscopy, dark field and transmission electron microscopy (TEM).

Unit Size
3/10 reactions

Optical Density
50 OD (when dissolved to a final volume of 100ul)

No.Particles/mL
5.8E+11 (when dissolved to a final volume of 100ul)

Shelf Life
If stored unopened and as specified, NHS-activated silver nanoparticles are stable for at least 3 months.

Storage
All components of this kit should be stored at -20°C.

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