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Absolute Mag™ Protein G Magnetic Particles, 2 µm(WHM-N007)

Datasheet

Absolute Mag™ Protein G Magnetic Particles, 2 µm (# WHM-N007) are affinity matrices for the small-scale isolation and purification of IgG types from different species. A truncated form of recombinant Protein G is covalently coupled to a nonporous paramagnetic particle. Specially, the beads can be regenerated without loss of binding capacity.

Application Notes
• Protein G exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep.• Specific antibodies can be chemically cross-linked to the Protein G coated surface to create a reusable immunoprecipitation particle, avoiding the co-elution of antibody with target antigen.• The particles can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants.
Particle Size
2 µm
Ligand
Protein G
Concentration
50% w/v
Storage Buffer
PBS, 0.02% NaN3, 0.1% BSA, 0.5% Tween 20, pH 7.4 (25°C)
Unit Size
1 mL
Type Magnetization
Paramagnetic
Sample Type
Serum, Ascites, Cell Culture Supernatants
Storage
4 °C

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