MagIso™ plasmidDNA Purification Kit (# WHK-C010) is a highly efficient tool for the isolation of plasmidDNA with magnetic silica particles. The plasmidDNA can be isolated directly from E.coli cultures. The pelleted bacteria are resuspended (Suspension Solution) and the E.coli cells are lysed by SDS/alkaline (Lysis Buffer). The Neutralization/Bead Buffer precipitates chromosomal DNA, denatured proteins, cellular debris and SDS which are removed from the sample by magnetic separation. The plasmidDNA binds to the magnetic silica particles under ethanolic conditions (Binding Buffer). The wash steps guarantee a clean plasmidDNA which is suitable for transfections or other biochemical applications. This kit is highly suitable for variety of automatization platforms since it require no centrifugation, vacuum filtration procedures.
