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Home / Beads Based Kits and Devices / DNA Extraction and Purification / MagIso™ Plasmid DNA Purification Kit

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MagIso™ Plasmid DNA Purification Kit(WHK-C010)

Datasheet

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MagIso™ plasmidDNA Purification Kit (# WHK-C010) is a highly efficient tool for the isolation of plasmidDNA with magnetic silica particles. The plasmidDNA can be isolated directly from E.coli cultures. The pelleted bacteria are resuspended (Suspension Solution) and the E.coli cells are lysed by SDS/alkaline (Lysis Buffer). The Neutralization/Bead Buffer precipitates chromosomal DNA, denatured proteins, cellular debris and SDS which are removed from the sample by magnetic separation. The plasmidDNA binds to the magnetic silica particles under ethanolic conditions (Binding Buffer). The wash steps guarantee a clean plasmidDNA which is suitable for transfections or other biochemical applications. This kit is highly suitable for variety of automatization platforms since it require no centrifugation, vacuum filtration procedures.

Application Notes

For isolation of plasmid DNA from E.coli with magnetic particles.

Kit Components

Kit Components.	Quantity
Lysis & Binding Buffer	15 mL
Wash Buffer I	30 mL
MagIso™DNA Particles	1.5 mL

Unit Size

10, 50, 250 preps from 1.5 mL of E. coli

Sample Type

E.coli Cultures

Cat. #	Product Name	Unit Size	Price
WHK-C001	MagIso™ Genomic DNA Purification Kit, Blood	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C003	MagIso™ Genomic DNA Purification Kit, Bacteria	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C005	MagIso™ Genomic DNA Purification Kit, Meat	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C006	MagIso™ Genomic DNA Purification Kit, Serum	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C007	MagIso™ Genomic DNA Purification Kit, Saliva	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C008	MagIso™ Genomic DNA Purification Kit, Tissue	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C011	MagIso™ Total RNA/DNA Purification Kit	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C013	MagIso™ PCR Cleanup Kit	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry
WHK-C014	MagIso™ Dye-terminator Removal Kit, Sequencing Cleanup	10, 50, 250 preps from 1.5 mL of E. coli	Online Inquiry