Home / Beads Based Kits and Devices / Cell Isolation / MagIso™ Pan Dendritic Cell Isolation Kit, Mouse
MagIso™ Pan Dendritic Cell Isolation Kit, Mouse(WHK-B011)

Datasheet

Mouse non dendritic cells are depleted by incubating the sample with the optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched classical and plasmacytoid dendritic cells are collected by decanting the liquid in a clean tube. These are cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. This kit may complement greatly protocols to further isolate classical or plasmacytoid Dendritic Cells by flow cytometry. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Mouse pan dendritic cells negative selection.
Designed for the isolation of untouched pan dendritic (classical and plasmacytoid) cells from lymphoid tissue.
Kit Components
Kit ComponentsQuantityStorage
Streptavidin magnetic nanoparticles200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Biotin-antibody mixture: Biotin anti- CD3ε, CD19, CD20, CD49b, CD64, CD105, CD335, Ly-6G, TCR γ/δ, TER-119200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing BSA and 0.05% sodium azide.
Unit Size
20 tests, 200 tests
Sample Type
Lymphoid tissue
Target Molecule
Pan Dendritic Cell
Reactivity
Mouse

Contact us to discuss your Service & Products Interested issues or request a quote

Telephone: Email: 1-631-624-4882 Please fill out the form below:
×

Contact Us

Company: Creative Diagnostics

Email:

Phone: 1-631-624-4882 (USA)

Phone: 44-161-818-6441 (Europe)

Fax: 1-631-938-8221

Distributors
Copyright © 2009 - Creative Diagnostics. All rights reserved.
Inquiry Basket