Home / Beads Based Kits and Devices / Cell Isolation / MagIso™ Pan B Cell Isolation Kit, Mouse
MagIso™ Pan B Cell Isolation Kit, Mouse(WHK-B013)

Datasheet

Mouse non B cells are depleted by incubating sample with the mouse Pan B cell isolation optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched Pan B cells are collected by decanting the liquid in a clean tube. These are cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, and phenotypic characterization. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Mouse pan B cells negative selection.
Designed for the isolation of untouched pan B cells from lymphoid tissues and designed for animal strains that express the CD90.2 allele (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, and NZB/-).
Kit Components
Kit ComponentsQuantityStorage
Streptavidin magnetic nanoparticles200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Biotin-antibody mixture: Biotin anti- CD90.2, CD11c, Gr-1, CD49b and TER-119/Erythroid200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing 0.3% BSA and 0.05% sodium azide.
Unit Size
20 tests, 200 tests
Sample Type
Lymphoid tissue
Reactivity
Mouse

Contact us to discuss your Service & Products Interested issues or request a quote

Telephone: Email: 1-631-624-4882 Please fill out the form below:
×

Contact Us

Company: Creative Diagnostics

Email:

Phone: 1-631-624-4882 (USA)

Phone: 44-161-818-6441 (Europe)

Fax: 1-631-938-8221

Distributors
Copyright © 2009 - Creative Diagnostics. All rights reserved.
Inquiry Basket