Home / Beads Based Kits and Devices / Cell Isolation / MagIso™ Pan B Cell Isolation Kit, Mouse Ⅱ
MagIso™ Pan B Cell Isolation Kit, Mouse Ⅱ(WHK-B012)

Datasheet

Mouse non-B cells are depleted by incubating the sample with the Mouse Pan B cell isolation optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched mouse total B cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Mouse pan B cells negative selection.
Designed for the isolation of untouched pan B cells from lymphoid tissues.
Kit Components
Kit ComponentsQuantityStorage
Streptavidin magnetic nanoparticles200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Biotin-antibody mixture: Biotin anti- CD3, CD4, CD8a, CD11c, CD49B, Ly-6G/Ly-6C (Gr-1), and TER-119200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing 0.3% BSA and 0.05% sodium azide.
Unit Size
20 tests, 200 tests
Sample Type
Lymphoid tissue
Reactivity
Mouse

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