Home / Magnetic Particles / Affinity Magnetic Particles / Oligo(dT) / Absolute Mag™ Oligo(dT)25 Magnetic Particles
Absolute Mag™ Oligo(dT)25 Magnetic Particles(WHM-L037)

Datasheet

Absolute Mag™ Oligo(dT)25 Magnetic Particles (# WHM-L037) are uniform superparamagnetic particles with oligo dT sequences covalently coupled to the particle surface. Relying on base-pairing between the poly A tail of messenger RNA and the oligo dT sequences, these particles are designed for the rapid isolation of highly purified, intact mRNA from eukaryotic total RNA or directly from crude extracts of cells, animal and plant tissues. And the isolated mRNA can be used directly in most downstream applications in molecular biology.

Application Notes
Absolute Mag™ Oligo(dT)25 Magnetic Particles are the ideal mRNA purification method prior to cDNA library construction. Use of these beads ensures the highest recovery and enrichment of the transcriptome. These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation. And the isolated mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis, cDNA library construction, RT-PCR, quantitative RT-PCR, RPA (Ribonuclease Protection Assay), subtractive hybridization, primer extension, SAGE, RACE, and others.
Particle Size
2.8 μm
Ligand
Oligo-dT
Concentration
5 mg/mL
Storage Buffer
PBS, 0.02% NaN3, pH 7.40
Unit Size
2 mL
Type Magnetization
Superparamagnetic
Binding Capacity
Up to 2 μg poly(A)+ RNA can be isolated per 200 μL (1 mg) of particles, depending on the tissue or cell type and the expression level of the mRNA. A typical mammalian cell contains about 10–30 pg of RNA of which 1–5% is mRNA. The total capacity per mL of particles is approx. 10 μg mRNA. If the same particles are reused for a total of 5 mRNA isolations (four regeneration cycles) the total capacity of 1 mL beads is up to 50 μg of mRNA.
Storage
Store at 2-8°C.
Shipping Condition
Room Temperature.
Shelf Life
36 months

Contact us to discuss your Service & Products Interested issues or request a quote

Telephone: Email: 1-631-624-4882 Please fill out the form below:
×

Contact Us

Company: Creative Diagnostics

Phone: 1-631-624-4882

Fax: 1-631-938-8221

Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Email:

Distributors
Copyright © 2009 - Creative Diagnostics. All rights reserved.
Inquiry Basket