Home / Beads Based Kits and Devices / Cell Isolation / MagIso™ Neutrophil Cell Isolation Kit, Mouse
MagIso™ Neutrophil Cell Isolation Kit, Mouse(WHK-B015)

Datasheet

Non-Neutrophil cells are depleted by incubating the sample with the optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched Neutrophils are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Mouse neutrophil cells negative selection.
Designed for the isolation of neutrophils from C57BL/6 bone marrow cells.
Kit Components
Kit ComponentsQuantityStorage
Streptavidin magnetic nanoparticles100 μL-10 tests/1 mL-100 tests2-8 °C
Biotin-antibody mixture: Biotin anti- CD4, CD5, B220, CD11c, CX3CR1, F4/80, CD117, CD244.2, TER-119/Erythroid100 μL-10 tests/1 mL-100 tests2-8 °C
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing 0.3% BSA and 0.05% sodium azide.
Unit Size
10 tests, 100 tests
Sample Type
C57BL/6 bone marrow cells
Reactivity
Mouse

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