Home / Beads Based Kits and Devices / Cell Isolation / MagIso™ Naïve B Cell Isolation Kit, Human
MagIso™ Naïve B Cell Isolation Kit, Human(WHK-B024)

Datasheet

Non naïve B cells are depleted by incubating the sample with the Human Naïve B cell isolation optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched human naive B cells are collected by decanting the liquid in a clean tube. These are cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Human naïve B cells negative selection.
Designed for the isolation of untouched Naïve B cells from peripheral blood mononuclear cells (PBMCs).
Kit Components
Kit ComponentsQuantityStorage
Streptavidin magnetic nanoparticles100 μL-20 tests/1 mL-200 tests2-8 °C
Biotin-antibody mixture: Biotin anti- CD2, CD3, CD16, CD27, CD36, CD43, CD235ab100 μL-20 tests/1 mL-200 tests2-8 °C
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing BSA and 0.05% sodium azide.
Unit Size
20 tests, 200 tests
Sample Type
Peripheral blood mononuclear cells (PBMCs)
Reactivity
Human

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