Absolute Mag™ NTA-Ni Iron Oxide Nanoparticles are water soluble iron oxide nanoparticles with lipid coating. These nanoparticles are covalently modified with strong metal-chelating nitrilotriacetic acid (NTA) groups and pre-charged with nickel. Their organic layers have about 1-2 nm thickness and zeta potential ranges from -10 mV to -30 mV. Their hydrodynamic size using a Zeta Trac (DLS) is about 3-8 nm larger than the inorganic core size measured with TEM.
Absolute Mag™ NTA-Ni iron oxide nanoparticles have a high affinity to proteins with histidine (His) tag, about 20-200 fold higher than non-His tagged proteins depending on the iron oxide nanoparticle core size. His-tagged proteins or other biomolecules can be easily attached to Absolute Mag™ NTA-Ni iron oxide nanoparticles and presented in a directional manner for enhanced signal-to-noise and reproducibility. The coating physiological lipids have excellent compatibility for in vivo studies. Absolute Mag™ NTA-Ni iron oxide nanoparticles are stable in most common buffers like borate, Tris, HEPES, PBS and MES buffers, as well as in cell culture medium.