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MagIso™ NK Cell Isolation Kit, Human(WHK-B023)

Datasheet

Non-natural killer (NK) cells are depleted by incubating the sample with the optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched NK cells (CD56+, CD3-) are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Human NK cells negative selection.
Designed for the isolation of untouched Natural Killer (NK) cells from peripheral blood mononuclear cells (PBMCs).
Kit Components
Kit ComponentsQuantityStorage
Streptavidin magnetic nanoparticles200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Biotin-antibody mixture: Biotin anti- CD14, CD15, CD3, CD4, CD279, CD19, CD36, CD123, CD235ab200 μL-20 tests/2x 1 mL-200 tests2-8 °C
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing BSA and 0.05% sodium azide.
Unit Size
20 tests, 200 tests
Sample Type
Peripheral blood mononuclear cells (PBMCs)
Reactivity
Human

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