Home / Magnetic Particles / Affinity Magnetic Particles / Antibody / Absolute Mag™ Human Anti-PE Magnetic Nanoparticles, 130 nm
Absolute Mag™ Human Anti-PE Magnetic Nanoparticles, 130 nm(WHM-B003)

Datasheet

Human PE labeled peripheral blood mononuclear cells (PBMCs) are either selected or depleted in a two-step separation process. The cells are first incubated with an anti-human PE-conjugated antibody, followed by incubating with directly Absolute Mag™ Human anti-PE Magnetic Nanoparticles (# WHM-B003). The PE+ magnetically labeled antibody is pulled out by the use of a magnetic separator. After collection or depletion of the PE+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Used as a two-step kit for the positive selection or depletion of PE labeled cells from peripheral blood mononuclear cells (PBMCs).
Kit Components
For 100 μL:
• 1 vial containing 250 µL of human Fc receptor blocking solution
• 1 vial containing 100 µL of human CD8 magnetic nanoparticles
For 1 mL:
• 1 vial containing 1 mL of human Fc receptor blocking solution
• 1 vial containing 1 mL of human CD8 magnetic nanoparticles
Particle Size
130 nm
Ligand
Anti-Human PE Antibody
Concentration
N/A
Storage Buffer
Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Unit Size
100 μL, 1 mL
Sample Type
Peripheral blood mononuclear cells
Target Molecule
CD3, CD4, CD8a, CD19, TCR Vγ9
Reactivity
Human
Storage
2-8 °C

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