Home / Beads Based Kits and Devices / Cell Isolation / MagIso™ Hematopoietic Progenitor Cell Isolation Kit, Mouse
MagIso™ Hematopoietic Progenitor Cell Isolation Kit, Mouse(WHK-B016)

Datasheet

Mouse non-hematopoietic stem/progenitor cells are enriched by incubating your sample with the optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched hematopoietic stem/progenitor cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. The enriched cells can be used for downstream applications including functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Mouse hematopoietic progenitor cells negative selection.
Designed for the isolation of untouched hematopoietic stem/progenitor cells (HSCs/HPCs) from mouse bone marrow.
Kit Components
For 10 tests:
• 100 µL of biotin-antibody mixture: biotin anti- CD3ε, CD45R/B220, Ly-6G/Ly-6C (Gr-1), CD11b, TER-119
• 100 µL streptavidin magnetic nanoparticles
For 100 tests:
• 1 mL of biotin-antibody mixture: biotin anti- CD3ε, CD45R/B220, Ly-6G/Ly-6C (Gr-1), CD11b, TER-119
• 1 mL streptavidin magnetic nanoparticles
Particle Size
130 nm
Ligand
Streptavidin
Storage Buffer
Biotin-antibody mixture: phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing 0.3% BSA and 0.05% sodium azide.
Unit Size
10 tests, 100 tests
Sample Type
Mouse bone marrow
Reactivity
Mouse
Storage
2-8 °C

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