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Absolute Mag™ Glutathione Magnetic Particles(WHM-L046)

Datasheet

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Absolute Mag™ Glutathione Magnetic Particles (# WHM-L046) are high-performance, superparamagnetic particles, providing a fast and convenient method for manual or automated purification of glutathione S-transferase (GST) fusion proteins from a bacteria, yeast or mammalian crude cell lysate. Glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding.

Application Notes

Absolute Mag™ Glutathione Magnetic Particles provide a fast and convenient method for manual or automated purification of glutathione S-transferase (GST) fusion proteins from a bacteria, yeast or mammalian crude cell lysate. These particles are simply added to a lysate containing the GST fusion protein. After incubation, non-bound proteins are removed by washing, and the GST fusion proteins are eluted using reduced glutathione. A magnetic stand is used for manual processing.

Particle Size

1-10 μm

Ligand

Glutathione

Concentration

25% v/v

Storage Buffer

20% ethanol

Unit Size

4 mL

Type Magnetization

Superparamagnetic (no magnetic memory)

Matrix

Crosslinked agarose

Binding Capacity

5-10 mg of GST fusion protein per 1mL of settled beads (i.e., 4mL of 25% slurry).

Target Molecule

GST

Storage

Store at 4°C. Do not freeze, dry or centrifuge.

Shipping Condition

Shipped with an ice pack.

Cat. #	Product Name	Unit Size	Price
WHM-G027	Absolute Mag™ Glutathione Magnetic Nanoparticles, Dextran Coated, 130 nm	4 mL	Online Inquiry
WHM-G042	Absolute Mag™ Glutathione Magnetic Nanoparticles, Dextran Coated, 250 nm	4 mL	Online Inquiry
WHM-S187	Absolute Mag™ Glutathione Magnetic Particles, 3.2 µm	4 mL	Online Inquiry