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Absolute Mag™ Glutathione Magnetic Particles(WHM-L046)

Datasheet

Absolute Mag™ Glutathione Magnetic Particles (# WHM-L046) are high-performance, superparamagnetic particles, providing a fast and convenient method for manual or automated purification of glutathione S-transferase (GST) fusion proteins from a bacteria, yeast or mammalian crude cell lysate. Glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding.

Application Notes
Absolute Mag™ Glutathione Magnetic Particles provide a fast and convenient method for manual or automated purification of glutathione S-transferase (GST) fusion proteins from a bacteria, yeast or mammalian crude cell lysate. These particles are simply added to a lysate containing the GST fusion protein. After incubation, non-bound proteins are removed by washing, and the GST fusion proteins are eluted using reduced glutathione. A magnetic stand is used for manual processing.
Particle Size
1-10 μm
Ligand
Glutathione
Concentration
25% v/v
Storage Buffer
20% ethanol
Unit Size
4 mL
Type Magnetization
Superparamagnetic (no magnetic memory)
Matrix
Crosslinked agarose
Binding Capacity
5-10 mg of GST fusion protein per 1mL of settled beads (i.e., 4mL of 25% slurry).
Target Molecule
GST
Storage
Store at 4°C. Do not freeze, dry or centrifuge.
Shipping Condition
Shipped with an ice pack.

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