1 µm fluorescent silica particles with carboxylic acid groups (COOH) on the surface. Fluorescence dyes are covalently bound in the silica matrix and availbale withgreen fluorescence (excitation: 485 nm, emission: 510 nm).
|Q: Could I know binding capacity of ssDNA to 1mg of these carboxyl silica particles?|
|A: The binding capacity needs to be determined experimentally for your specific case, since the size of the protein and its conformation can have a large influence and may lead to incomplete conjugation to the activated carboxy-groups.|
|Q: To avoid non-specific binding, is there blocking protocols after conjugation of ssDNA? (such as BSA blocking)?|
|A: For blocking, we recommend to use simple glycine.|
|Q: If I use blue or red fluorescent carboxyl silica particle with this green carboxy silica particle, will Foster Resonance Energy Transfer happen?|
|A: Foster Resonance Energy Transfer should not be an issue because the fluorescence dyes are covalently embedded in the SiO2-matrix.|