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Absolute Mag™ Chitin Magnetic Particles(WHM-N013)

Datasheet

Absolute Mag™ Chitin Magnetic Particles (# WHM-N013) are paramagnetic microparticles designed for the small-scale isolation of target proteins fused to a chitin binding domain (CBD). The magnetite core of chitin particles permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants, after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.

Application Notes
The magnetite core of chitin particles permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants, after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.
Particle Size
50–70 µm
Ligand
Chitin
Concentration
1.0% v/v
Storage Buffer
20% ethanol in water
Unit Size
5 mL
Type Magnetization
Paramagnetic
Binding Capacity
100 μL of Absolute Mag™ Chitin Magnetic Particles will bind 30–50 μg of CBD fusion protein.
Storage
Store at 4°C. Do not freeze.

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