Home / Beads Based Kits and Devices / Organelles Isolation / MagIso™ CD44 Exosome Isolation Kit for FACS
MagIso™ CD44 Exosome Isolation Kit for FACS(WHK-S005)

Datasheet

MagIso™ CD44 Exosome Isolation Kit for FACS (# WHK-S005) is for the selective capture and flow sorting to purify distinct subpopulations of exosomes, based on a particular surface marker. You will first enrich for all exosomes. The isolated exosomes are then resuspended and bound to the magnetic particles for specific capture and subsequent FACS analysis and sorting. The MagIso™ CD44 Exosome Isolation Kit for FACS is modular, thus you can select from various pre-validated capture antibody kits, or utilize your own biotinylated antibody corresponding to the exosome surface marker specific for the exosomes of interest in your model system. We has thoroughly tested a variety of capture antibodies that work quite well to flow-sort exosomes from either serum or cell culture samples.

Application Notes
Exosome Isolation for FACS
Kit Components
Kit ComponentsQuantityStorage
9.1 μm Streptavidin Magnetic Particles400μL (10 mg/mL)4°C. DO NOT FREEZE.
CD44-biotin Capture Antibody (PBS)100 μL (100 ng/μL)4°C
Particle Wash Buffer60 mL4°C
Exosome Stain Buffer3 mL4°C
FITC Exosome Stain100 μL4°C
Exosome Elution Buffer3 mL4°C
Unit Size
10 reactions
Number of Particles
1.6E+7 particles/mL
Sample Type
Concentrated Exosomes from media

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