MagIso™ CD4 T Cell Isolation Kit, Human

(WHK-B001)

Non CD4+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanoparticles. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Antibody Array
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Datasheet

Overview


Application Notes
This kit is designed for the isolation of untouched CD4+ T cells from peripheral blood mononuclear cells (PBMCs).

Kit Components
For 10 tests:
• 100 µL of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD11b, CD16, CD14, CD19, CD20, CD36, CD56, TCR-γδ, CD123, CD235ab (Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2)
• 100 µL Streptavidin Nanoparticles (Aqueous solution containing BSA and 0.05% sodium azide)

Isolation Method
Negative Selection

Sample Type
Peripheral Blood Mononuclear Cells (PBMCs)

Species
Human

Storage
Stored undiluted at 2-8°C.

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