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MagIso™ CD3 T Cell Isolation Kit, Mouse(WHK-B007)

Datasheet

Non CD3+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanoparticles. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD3+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Application Notes
This kit is designed for the isolation of untouched CD3+ T cells from lymphoid tissues.
Kit Components
For 10 tests:
• 100 µL of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD4, CD11b, CD11c, CD19, CD24, CD45/B220, CD49b, CD105, I-A/I-E (MHC II), TER-119/Erythroid, TCR-γδ (Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2)
• 100 µL Streptavidin Nanoparticles (Aqueous solution containing BSA and 0.05% sodium azide)

Unit Size
10, 100 tests
Species Reactivity
Mouse
Isolation Method
Negative Selection
Sample Type
Lymphoid Tissues
Target Cell
CD3+ T cells
Storage
Stored undiluted at 2-8°C.

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