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MagIso™ CD3 Cell Isolation Kit, Mouse(WHK-B009)


Mouse CD3+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse CD3 antibody followed by streptavidin magnetic nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator. After collection of the CD3+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Clone 17A2 recognizes ε/γ (but not ε/δ) of the CD3 complex. It can induce T cell activation. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems.

Application Notes
Mouse CD3 positive selection.
Designed for the positive selection or depletion of mouse CD3+ cells from lymphoid tissue.
Kit Components
For 20 tests:
• 1 vial containing 200 µL of biotin anti-mouse CD3 antibody (clone 17A2)
• 1 vial containing 200 µL streptavidin magnetic nanoparticles
For 200 tests:
• 2 vials containing 1mL each of biotin anti-mouse CD3 antibody (clone 17A2)
• 2 vials containing 1mL each of streptavidin magnetic nanoparticles
Particle Size
130 nm
Storage Buffer
Biotin-antibody: phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin magnetic nanoparticles: aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Unit Size
20 tests, 200 tests
Sample Type
Lymphoid tissue
Target Molecule
2-8 °C

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