{"id":84,"date":"2020-02-13T01:57:14","date_gmt":"2020-02-13T06:57:14","guid":{"rendered":"https:\/\/www.cd-bioparticles.com\/blog\/?p=84"},"modified":"2020-02-13T01:57:14","modified_gmt":"2020-02-13T06:57:14","slug":"common-misunderstandings-in-nucleic-acid-extraction-by-magnetic-beads","status":"publish","type":"post","link":"https:\/\/www.cd-bioparticles.com\/blog\/applications\/common-misunderstandings-in-nucleic-acid-extraction-by-magnetic-beads\/","title":{"rendered":"Common Misunderstandings in Nucleic Acid Extraction by Magnetic Beads"},"content":{"rendered":"\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" src=\"\/blog\/wp-content\/uploads\/2020\/02\/Common-Misunderstandings-in-Nucleic-Acid-Extraction-by-Magnetic-Beads-1-1024x683.png\" alt=\"\" class=\"wp-image-85\" srcset=\"\/blog\/wp-content\/uploads\/2020\/02\/Common-Misunderstandings-in-Nucleic-Acid-Extraction-by-Magnetic-Beads-1-1024x683.png 1024w, \/blog\/wp-content\/uploads\/2020\/02\/Common-Misunderstandings-in-Nucleic-Acid-Extraction-by-Magnetic-Beads-1-300x200.png 300w, \/blog\/wp-content\/uploads\/2020\/02\/Common-Misunderstandings-in-Nucleic-Acid-Extraction-by-Magnetic-Beads-1-768x512.png 768w, \/blog\/wp-content\/uploads\/2020\/02\/Common-Misunderstandings-in-Nucleic-Acid-Extraction-by-Magnetic-Beads-1-120x80.png 120w, \/blog\/wp-content\/uploads\/2020\/02\/Common-Misunderstandings-in-Nucleic-Acid-Extraction-by-Magnetic-Beads-1.png 1090w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>Compared\nwith the traditional chloroform isoamyl alcohol extraction method and spin\ncolumn kit method, the use of biological magnetic beads for <a href=\"https:\/\/www.cd-bioparticles.com\/product\/dna-extraction-and-purification-list-8.html\">nucleic\nacid extraction<\/a> is a novel method for nucleic acid\nextraction. However, there are still many people who don&#8217;t fully understand\nthis method. There are also some misunderstandings in the process of purifying\nnucleic acids by magnetic beads.<\/p>\n\n\n\n<p>Myth 1:\nThe more magnetic beads are used, the better the extraction effect<\/p>\n\n\n\n<p>Many\npeople like to increase the number of magnetic beads when the extraction\nresults are not good. They think that adding more magnetic beads can adsorb\nmore nucleic acids. But this is not always the case.<\/p>\n\n\n\n<p>The\nmain feature of magnetic beads is that they can be dispersed in a liquid, and\ncan be separated from the liquid phase in a solid-state under the action of an\nexternal magnetic field. In any reagent system, the ratio of magnetic beads to\nliquid should have a certain threshold. Beyond a certain ratio, too many\nmagnetic beads will lose their dispersion characteristics because they cannot\nbe uniformly dispersed in the liquid, which also makes it impossible to fully\nincrease the efficiency of nucleic acid, magnetic beads, and liquid contact\nduring the washing process. Excessive magnetic beads will also adsorb more\nimpurities, making it more difficult to remove impurities. Moreover, too many\nmagnetic beads will adsorb protease, lysozyme and other functional components\nthat play a major role in the liquid system, resulting in low extraction\nefficiency of the kit. Many times, when the extraction effect is not good,\nreducing the number of magnetic beads used is the best way to improve the\nextraction effect.<\/p>\n\n\n\n<p>Under\nnormal circumstances, the amount of reference magnetic beads given by the kit\nis slightly excessive. Therefore, it is not necessary to increase the amount of\nmagnetic beads to improve the adsorption efficiency. However, if it is\ndetermined that the extraction effect is not good due to the insufficient\namount of magnetic beads. Then, it is possible to improve the extraction effect\nby increasing the number of magnetic beads within a certain range.<\/p>\n\n\n\n<p>Myth 2:\nThe more reagents are used, the better the extraction effect<\/p>\n\n\n\n<p>Poor\nlysing effect? Add more lysate. Poor washing effect? Add more wash solution.\nThis is the inertia of many customers in using kits.<\/p>\n\n\n\n<p>However,\nfor the magnetic bead method, each increase in the volume of liquid reduces the\nprobability of collision of magnetic beads, and reducing the probability of\ncollision of magnetic beads will cause a large decrease in the adsorption\nefficiency. So in many cases, although adding lysis buffer and washing buffer\ncan indeed enhance the lysis and enhance the washing effect, the core of\nmagnetic bead extraction is the adsorption efficiency of the magnetic bead for\nnucleic acid. A lower collision between magnetic beads results in the lower\nextraction effect. Therefore, simply increasing the amount of reagent used to\nimprove the extraction effect may not be completely effective.<\/p>\n\n\n\n<p>Myth 3:\nThe more washing times, the better the extraction effect<\/p>\n\n\n\n<p>When\nthere are too many impurities in the extracted nucleic acid, the user will\nconsider performing more washing to obtain more pure nucleic acid. Increasing\nthe number of washings is indeed beneficial for the purification of nucleic\nacids, but it is considered that a certain amount of nucleic acids is lost with\neach washing, and the possibility of nucleic acid fragmentation and hydrolysis\nis increased. Therefore, the number of washing times should be controlled at 2\nto 4 times.<\/p>\n\n\n\n<p>Myth 4:\nThe more samples you add, the better the extraction effect<\/p>\n\n\n\n<p>When\nthe sample is not fresh enough or the nucleic acid content itself is very\nsmall, the nucleic acid extraction is often not good. At this time, many people\nwill increase the amount of nucleic acid extraction by adding samples.<\/p>\n\n\n\n<p>But\nsimply increasing the sample size sometimes introduces excessive impurities. In\naddition, exceeding the lysing capacity of the lysis buffer will also reduce\nthe extraction efficiency, so it is not recommended to increase the amount of\nextraction by simply increasing the sample size.<\/p>\n\n\n\n<p>If the\nextraction volume is indeed too low due to insufficient sample volume, it is\nrecommended to go through the enrichment or concentration step before starting\nthe extraction during pretreatment. Or increasing the intensity of lysis to\nexpose more nucleic acids is also an alternative.<\/p>\n\n\n\n<p>Myth 5:\nIf a certain kind of magnetic bead is good, it should work well in all tests<\/p>\n\n\n\n<p>There\nare various types of magnetic beads, with different particle sizes, different\ndispersions, different magnetic response times, different coating base\nmatrices, different modified functional groups on the surface, different\ncoating densities, and different functional arm lengths, all of which will lead\nto different characteristics of magnetic beads. Therefore, different magnetic\nbeads adapt to different experiments and systems.<\/p>\n\n\n\n<p>Some\nmagnetic beads show higher adsorption efficiency in constant nucleic acid\nextraction, and some magnetic beads are more suitable for trace nucleic acid\nextraction. Some magnetic beads are suitable for acidic series reagent system,\nsome magnetic beads are suitable for alkaline series reagent system. Some\nmagnetic beads have a good magnetic response but fast sedimentation speed,\nwhich is more suitable for magnetic rod type automatic extraction instrument;\nsome magnetic beads have slow sedimentation speed but long magnetic response\ntime, and are more suitable for pipette type automatic extraction instrument.<\/p>\n\n\n\n<p>Few\nmagnetic beads are suitable for all experimental situations. Except for fixed\nreagent kits, in most cases, a series of adjustments must be made to the\nmagnetic beads and reagent system.\n\nIn addition to\nmagnetic beads and kits, CD Bioparticles also provides <a href=\"https:\/\/www.cd-bioparticles.com\/s\/Beads-Based-Kits-Development_30.html\">beads\nbased kits development<\/a> services, including helping clients adjust the amount of magnetic beads\nand the details of reagents, so that clients can find a more suitable system to\nobtain the best experimental results.\n\n\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Compared with the traditional chloroform isoamyl alcohol extraction method and spin column kit method, the use of biological magnetic beads<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[4,18],"class_list":["post-84","post","type-post","status-publish","format-standard","hentry","category-applications","tag-magnetic-particles","tag-nucleic-acid-extraction"],"_links":{"self":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/84","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/comments?post=84"}],"version-history":[{"count":1,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/84\/revisions"}],"predecessor-version":[{"id":86,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/84\/revisions\/86"}],"wp:attachment":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/media?parent=84"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/categories?post=84"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/tags?post=84"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}