{"id":78,"date":"2020-01-01T01:51:51","date_gmt":"2020-01-01T06:51:51","guid":{"rendered":"https:\/\/www.cd-bioparticles.com\/blog\/?p=78"},"modified":"2020-01-01T01:51:51","modified_gmt":"2020-01-01T06:51:51","slug":"aunps-showed-difference-of-permeable-capacity-by-size-of-aunp-in-the-tumorous-brain-tissue","status":"publish","type":"post","link":"https:\/\/www.cd-bioparticles.com\/blog\/nanoparticles\/aunps-showed-difference-of-permeable-capacity-by-size-of-aunp-in-the-tumorous-brain-tissue\/","title":{"rendered":"AuNPs Showed Difference of Permeable Capacity by Size of AuNP in The Tumorous Brain Tissue"},"content":{"rendered":"\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"506\" src=\"\/blog\/wp-content\/uploads\/2020\/01\/AuNPs-Showed-Difference-of-Permeable-Capacity-1-1024x506.png\" alt=\"\" class=\"wp-image-79\" srcset=\"\/blog\/wp-content\/uploads\/2020\/01\/AuNPs-Showed-Difference-of-Permeable-Capacity-1-1024x506.png 1024w, \/blog\/wp-content\/uploads\/2020\/01\/AuNPs-Showed-Difference-of-Permeable-Capacity-1-300x148.png 300w, \/blog\/wp-content\/uploads\/2020\/01\/AuNPs-Showed-Difference-of-Permeable-Capacity-1-768x379.png 768w, \/blog\/wp-content\/uploads\/2020\/01\/AuNPs-Showed-Difference-of-Permeable-Capacity-1.png 1047w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n\n\n<p>When\ndelivering nanoparticle drugs to the brain, the blood-brain barrier (BBB)\nlimits the drug transmission very much. Thus, researchers have made great\nefforts to overcome this issue to improve the efficiency of drug delivery (e.g.\nthe efficiency of nanoparticle drug delivery to cancer cells in tumor tissue\nthrough BBB). Recently, a research group from Gachon University have found that\nthere is a difference of permeable capacity by size of <a href=\"https:\/\/www.cd-bioparticles.com\/product\/fluorescent-gold-nanoparticles-list-177.html\">gold\nnanoparticle<\/a> (AuNP) in the tumorous brain tissue (no difference in\nthe normal brain tissue).<\/p>\n\n\n\n<p>Findings\nfrom the new study were published recently in Journal of Drug Targeting through\nan article titled \u201cInvestigation on the effect of nanoparticle size on the\nblood\u2013brain tumour barrier permeability by in situ perfusion via internal\ncarotid artery in mice\u201d.<\/p>\n\n\n\n<p>As the\nmost important limiting factor in nanoparticle drug delivery to the brain, the\nBBB is mainly composed of the following cell types: the brain capillary\nendothelial cells (BCECs), pericytes, astrocytes, and neurons. Among them,\nBCECs with several specific characteristics contribute the most to the BBB. For\nexample, there are continuous tight junctions between the BCECs, which prevents\nparacellular transport of substances from the blood to the brain. While in brain\ntumors, the BBB in the core of a tumor site is more compromised than in the\nsurrounding area and the drug distribution is more restricted than that in\nother organ tumors. AuNPs are with relatively low cytotoxicity, high water\nsolubility in target cells, easily modified surfaces and tunable light\nabsorption peaks, making them ideal for diagnostic imaging agents or\ntherapeutic agents for experimental genes and drug delivery and photothermal\ntherapy. AuNP-based drug delivery systems have been widely used in the\ndevelopment of cancer chemotherapy due to their high efficacy, low toxicity,\nhigh biocompatibility, and customizable designs. In this study, Ji Hee Kang\n(from Gachon Institute of Pharmaceutical Sciences, Gachon University)\ninvestigated the size-dependent AuNP (purchased from <a href=\"https:\/\/www.cd-bioparticles.com\/\">CD Bioparticles<\/a>)\npermeability across the blood-brain tumor barrier (BBTB) through internal\ncarotid artery (ICA) perfusion in orthotopic-glioblastoma multiforme (GBM) mice\nby using LSCM for the development of glioblastoma multiforme therapy.<\/p>\n\n\n\n<p>They\nused a mouse model of GBM, which was established by intracranial implantation\nof luciferase-expressing human glioblastoma U87MG cells. AuNPs coated with\nPEG-Alexa 633 were 10, 50, and 100 nm in size and were perfused via ICA of the\nGBM mice. In normal brain tissue, drugs only permeated across the BBB by\npassive diffusion while in brain tumors, there was an increasing permeability\nbetween microvessels. Then, the distribution of AuNP in these two tissues was\nobserved by confocal microscopy. <\/p>\n\n\n\n<p>They found that \u201c The permeation of AuNP across the brain endothelial cells is dependent on the size of the nanoparticles. Only 10 nm nanoparticles successfully permeated and spread in the brain tumor, with over 2.3-fold higher number of fluorescence signal that of either 50 or 100 nm nanoparticles\u201d and \u201cThe brain section perfused with 10, 50 and 100 nm AuNP showed similar number of blood vessels. All sizes of AuNP were similarly distributed, but the number of AuNP located near the blood vessels was increased with the nanoparticle size suggesting the size-dependent distribution of AuNP in normal brain. The number of AuNP distributed around blood vessels in the normal brain tissue was less than that in the brain tumor tissue. 10 nm AuNP was widely distributed in the brain tissue, whereas 50 and 100 nm AuNPs were aggregately located along the blood vessels.\u201d Thus, small nanoparticles (less than 50 nm) were more efficiently permeated from the blood into the brain, distributing across the brain tumor site. <\/p>\n\n\n\n<p>In conclusion, Kang and his colleagues successfully showed the size-dependent permeability of AuNPs in brain tumor tissue. Moreover, the permeable capacity of AuNPs in the tumorous brain tissue varies by size and no difference showed in the normal brain tissue. Their research presented us with a better understanding of the permeability of nanoparticles across the BBTB and may significantly promote the development of nanoparticle-based drug delivery systems that target brain tumors. <\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\"><p> Reference: <\/p><p>Kang, J. H., Cho, J., &amp; Ko, Y. T. (2019). Investigation on the effect of nanoparticle size on the blood\u2013brain tumour barrier permeability by in situ perfusion via internal carotid artery in mice. <em>Journal of drug targeting<\/em>, 27(1), 103-110. <\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>When delivering nanoparticle drugs to the brain, the blood-brain barrier (BBB) limits the drug transmission very much. Thus, researchers have<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[17,15,8,16],"class_list":["post-78","post","type-post","status-publish","format-standard","hentry","category-nanoparticles","tag-bbb","tag-fluorescent","tag-gold-nanoparticles","tag-tumor"],"_links":{"self":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/78","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/comments?post=78"}],"version-history":[{"count":1,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/78\/revisions"}],"predecessor-version":[{"id":80,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/78\/revisions\/80"}],"wp:attachment":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/media?parent=78"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/categories?post=78"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/tags?post=78"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}