{"id":264,"date":"2021-04-19T03:17:48","date_gmt":"2021-04-19T08:17:48","guid":{"rendered":"https:\/\/www.cd-bioparticles.com\/blog\/?p=264"},"modified":"2021-04-19T03:17:48","modified_gmt":"2021-04-19T08:17:48","slug":"quantum-dots-technology-in-fluorescent-labeling","status":"publish","type":"post","link":"https:\/\/www.cd-bioparticles.com\/blog\/applications\/quantum-dots-technology-in-fluorescent-labeling\/","title":{"rendered":"Quantum Dots Technology in Fluorescent Labeling"},"content":{"rendered":"\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"682\" src=\"\/blog\/wp-content\/uploads\/2021\/04\/Quantum-Dots-Technology-in-Fluorescent-Labeling-1024x682.jpg\" alt=\"\" class=\"wp-image-265\" srcset=\"\/blog\/wp-content\/uploads\/2021\/04\/Quantum-Dots-Technology-in-Fluorescent-Labeling-1024x682.jpg 1024w, \/blog\/wp-content\/uploads\/2021\/04\/Quantum-Dots-Technology-in-Fluorescent-Labeling-300x200.jpg 300w, \/blog\/wp-content\/uploads\/2021\/04\/Quantum-Dots-Technology-in-Fluorescent-Labeling-768x511.jpg 768w, \/blog\/wp-content\/uploads\/2021\/04\/Quantum-Dots-Technology-in-Fluorescent-Labeling-120x80.jpg 120w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n\n\n<p>Quantum dot technology has been widely used in our daily life, including energy, solar cells, displays, biomedical and biological applications. Especially in the field of biomedicine, the application of <a href=\"https:\/\/www.cd-bioparticles.com\/product\/quantum-dots-list-169.html\">quantum dot<\/a> technology in fluorescence labeling has greatly improved the imaging quality of cells and <em>in vivo<\/em>. <\/p>\n\n\n\n<p>Highly\nsensitive analysis and detection of important biomolecules such as proteins,\nnucleic acids, and peptides <em>in vivo<\/em> and in the process of life is an\nimportant problem in the field of life science research. Exploring and\ndeveloping high-sensitivity analysis and detection methods has always been the\ndirection of researchers in this field. Fluorescence analysis is one of the\nmost important methods in biological research, and its detection sensitivity\nlargely depends on the luminescence intensity and photochemical stability of\nthe label. The currently used organic fluorescent dyes have insurmountable\nfluorescent performance defects: narrow excitation spectrum, wide emission\nspectrum and tailing, easy photo-bleaching, and their own toxicity, which\ngreatly limits their application in life sciences.<\/p>\n\n\n\n<p> The attention and research on semiconductor fluorescent nanomaterials quantum dots (Quantum dots, QDs) have begun in the late 1970s. As a typical representative of inorganic fluorescent materials, QDs belong to artificially prepared nano-semiconductor materials. The particle size ranges from 1-20 nm and can have different fluorescent colors according to their size. Common types include type II-VI (CdTe, <a href=\"https:\/\/www.cd-bioparticles.com\/product\/cdse-quantum-dots-list-200.html\">CdSe<\/a>, CdS), type II-V (InP, InAs), type I-III-VI2 (<a href=\"https:\/\/www.cd-bioparticles.com\/product\/cis-se-cigs-se-czts-se-quantum-dots-list-210.html\">CuInS2<\/a>, AgInS2), and type IV-VI (<a href=\"https:\/\/www.cd-bioparticles.com\/product\/pbse-quantum-dots-list-250.html\">PbSe<\/a>). These quantum dots play a very important role in various medical applications such as bioimaging, biosensor, and drug delivery. <\/p>\n\n\n\n<p>Biomedical\napplications of quantum dots<\/p>\n\n\n\n<p>In 1998,\nAlivisatos and Nie innovatively solved the problem of biocompatibility after\nquantum dot labeling and realized the combination of biological macromolecules\nand quantum dots. Then quantum dots began to be widely used in a variety of\nbiotechnologies including DNA detection, immunofluorescence, and cell biology.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><em>In vivo<\/em>\napplications<\/li><\/ul>\n\n\n\n<p>Nie et\nal. found that using CdSe quantum dots modified with thioglycolic acid and\nchemically cross-linked with transferrin, transferrin can enter the cell\ninterior and track the donor-acceptor reaction. Nafiujjaman&#8217;s research shows\nthat the diameter of the new Cl-GQDs-N quantum dots is about 30nm. <em>In vitro<\/em>\nexperiments have shown that QDs have no toxic effects on both cancer cells and\nnormal cells, and have promising applications in cell imaging.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Bioimaging<\/li><\/ul>\n\n\n\n<p>NIR\nlight has small background interference and large penetration depth, making NIR\nquantum dots incomparable advantages in the imaging of living animals. Akerman\net al. confirmed that after intravenous injection of biomarker labeled quantum\ndots in mice, quantum dots can specifically target the specific tissues of mice\nand be successfully imaged.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><em>In vitro<\/em>\ndiagnostics<\/li><\/ul>\n\n\n\n<p>Quantum\ndots are used in <em>in vitro<\/em> diagnostics and are mainly used as fluorescent\nprobes to directly or indirectly detect biomolecules. Azmi et al. reported a\nnew type of fluorescent biosensor in a 96-well microplate format, encapsulated\nwith CdS quantum dots (QDs)-uricase\/horseradish peroxidase (HRP) enzyme. The\nuse of quantum dots as a fluorescent indicator reveals the fluorescent signal\nof the uricase\/HRP enzymatic reaction system in the presence of uric acid. The\nsensor has been successfully applied to the detection of uric acid in human\nurine, and the result is comparable to the assay kit.<\/p>\n\n\n\n<p>As a new generation of fluorescent nano-markers, quantum dots are a field with extremely broad development prospects in the application of life sciences. With the continuous improvement of quantum dot synthesis and modification technology, the regulation of the size, structure, performance, and dispersion of quantum dots will gradually be realized, and it is expected to be applied to mass screening in the field of proteomics and genomics. The unique fluorescence properties of quantum dots make it possible to study a series of life activities in the dynamic process of living cells.<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\"><p>References:<br> Bruchez, M., Moronne, M., Gin, P., Weiss, S., &amp; Alivisatos, A. P. (1998). Semiconductor nanocrystals as fluorescent biological labels. Science, 281(5385), 2013-2016.<br> Chan, W. C., &amp; Nie, S. (1998). Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science, 281(5385), 2016-2018.<br> Nafiujjaman, M., Joon, H., Kwak, K. S., &amp; Lee, Y. K. (2018). Synthesis of nitrogen-and chlorine-doped graphene quantum dots for cancer cell imaging. Journal of nanoscience and nanotechnology, 18(6), 3793-3799.<br> \u00c5kerman, M. E., Chan, W. C., Laakkonen, P., Bhatia, S. N., &amp; Ruoslahti, E. (2002). Nanocrystal targeting in vivo. Proceedings of the National Academy of Sciences, 99(20), 12617-12621.<br> Azmi, N. E., Rashid, A. H. A., Abdullah, J., Yusof, N. A., &amp; Sidek, H. (2018). Fluorescence biosensor based on encapsulated quantum dots\/enzymes\/sol-gel for non-invasive detection of uric acid. Journal of luminescence, 202, 309-315. <\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>Quantum dot technology has been widely used in our daily life, including energy, solar cells, displays, biomedical and biological applications.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7,31],"tags":[15,10],"class_list":["post-264","post","type-post","status-publish","format-standard","hentry","category-applications","category-quantum-dots","tag-fluorescent","tag-quantum-dots"],"_links":{"self":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/264","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/comments?post=264"}],"version-history":[{"count":1,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/264\/revisions"}],"predecessor-version":[{"id":266,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/posts\/264\/revisions\/266"}],"wp:attachment":[{"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/media?parent=264"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/categories?post=264"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cd-bioparticles.com\/blog\/wp-json\/wp\/v2\/tags?post=264"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}