MagIso™ Plasmid DNA Purification Kit

(WHK-C010)

MagIso™ plasmidDNA Purification Kit (# WHK-C010) is a highly efficient tool for the isolation of plasmidDNA with magnetic silica particles. The plasmidDNA can be isolated directly from E.coli cultures. The pelleted bacteria are resuspended (Suspension Solution) and the E.coli cells are lysed by SDS/alkaline (Lysis Buffer). The Neutralization/Bead Buffer precipitates chromosomal DNA, denatured proteins, cellular debris and SDS which are removed from the sample by magnetic separation. The plasmidDNA binds to the magnetic silica particles under ethanolic conditions (Binding Buffer). The wash steps guarantee a clean plasmidDNA which is suitable for transfections or other biochemical applications. This kit is highly suitable for variety of automatization platforms since it require no centrifugation, vacuum filtration procedures.


Datasheet

Overview


Application Notes
For isolation of plasmid DNA from E.coli with magnetic particles.

Kit Components
For 10 preps:
• 2 mg RNase A (lyophilized)
• 2 mL Lysis Buffer
• 2 mL Neutralization Buffer
• 1.5 mL MagIso™-plasmidDNA Particles

Sample Type
E.coli Cultures

Storage
Store the kit at room temperature.

Cat.# Product Name Size Price
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WHK-C003 MagIso™ Genomic DNA Purification Kit, Bacteria 15, 100, 500 preps from <br/>10^9 bacteria Inquiry
WHK-C005 MagIso™ Genomic DNA Purification Kit, Meat 15, 100, 500 preps from 100 mg meat Inquiry
WHK-C006 MagIso™ Genomic DNA Purification Kit, Serum 15, 100, 500 preps per 100 µL serum Inquiry
WHK-C007 MagIso™ Genomic DNA Purification Kit, Saliva 15, 100, 500 preps from 500 µL saliva Inquiry
WHK-C008 MagIso™ Genomic DNA Purification Kit, Tissue 15, 100, 500 preps from 100 mg tissue Inquiry
WHK-C011 MagIso™ Total RNA/DNA Purification Kit 15, 100, 500 preps from 1.5 mL of E. coli Inquiry
WHK-C013 MagIso™ PCR Cleanup Kit
1 x 96, 5 x 96, 10 x 96 preps Inquiry
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Contact Us
Company: Creative Diagnostics
Phone: 1-631-624-4882
Fax: 1-631-938-8221
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
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