DiagNano™ Gold Nanoparticle Covalent Conjugation Kit, 5 nm

(GCK-5)

Kit for one-step covalent conjugation of proteins and other amine containing ligands to 5 nm gold nanoparticles. Contains enough reagents for conjugation. 200 ug of protein can be conjugated in each reaction.

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Datasheet

Overview


Diameter
5 nm

Absorption Max
515-520 nm

Kit Components
5nm NHS-Activated Gold Nanoparticles (lyophilized), Protein Resuspension Buffer, Reaction Buffer, Quencher Solution.

Functional Group
NHS

Surface Coating
NHS-ester with a PEG5000 linker (~1 NHS group/nm2)

Application Notes
Ideal for development of protein gold conjugates for use in applications such as blotting, lateral flow assays, microscopy and transmission electron microscopy (TEM).

Unit Size
3/10 reactions

Optical Density
OD=20 (when dissolved to a final volume of 100ul)

No.Particles/mL
1.1E+14 (when dissolved to a final volume of 100ul)

Shelf Life
The products are stable for at least 3 months if stored as specified.

Storage
All components of this kit should be stored at -20°C.

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