Streptavidin attached Quantum dots Conjugation Kit, excitation at 405 nm, emission at 655 nm. Streptavidin covalently attached to the inner amphiphilic coating without a PEG linker, for binding biotinylated probes or other biomolecules.
|Q: What does the kit contain in terms of quantity of materials and how much antibodies that can be conjugated?|
|A: The DiagNano™ Streptavidin Quantum Dots Conjugation Kit provide Qdot streptavidin conjugate as 250 µL of a 2 µM solution. The kit also includes 30 mL of Qdot incubation buffer.|
|Q: Number of streptavidins per QD|
|A: The average number of streptavidins is 6 to 8 per quantum dot. This is an estimate based on a microtiter plate binding assay using biotinylated oligos as a target. This is only an approximation, and the number will vary slightly within the batch of the material. We don't have a method for determining the exact number of streptavidins per quantum dot.|
We don't have specific buffer recommendations for Qdot streptavidin conjugates, but the conjugates are stable at stock concentrations in nearly any physiological buffer. The most prominent warning is to not store diluted working solutions.