MagIso™ CD8 T Cell Isolation Kit, Mouse

(WHK-B004)

Non CD8+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanoparticles. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD8+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Antibody Array
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Datasheet

Overview


Application Notes
This kit is designed for the isolation of untouched CD8+ T cells from lymphoid tissues.

Kit Components
For 10 tests:
• 100 µL of Biotin-Antibody Cocktail: Biotin anti- CD4, CD11b, CD11c, CD19, CD24, CD45/B220, CD49b, CD105, I-A/I-E (MHC II), TER-119/Erythroid, TCR-γδ (Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2)
• 100 µL Streptavidin Nanoparticles (Aqueous solution containing BSA and 0.05% sodium azide)

Isolation Method
Negative Selection

Sample Type
Lymphoid Tissues

Species
Mouse

Storage
Stored undiluted at 2-8°C.

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