MagIso™ CD3 T Cell Isolation Kit, Mouse

(WHK-B007)

Non CD3+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanoparticles. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD3+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.


Datasheet

Overview


Application Notes
This kit is designed for the isolation of untouched CD3+ T cells from lymphoid tissues.

Kit Components
For 10 tests:
• 100 µL of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD4, CD11b, CD11c, CD19, CD24, CD45/B220, CD49b, CD105, I-A/I-E (MHC II), TER-119/Erythroid, TCR-γδ (Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2)
• 100 µL Streptavidin Nanoparticles (Aqueous solution containing BSA and 0.05% sodium azide)


Isolation Method
Negative Selection

Sample Type
Lymphoid Tissues

Species
Mouse

Storage
Stored undiluted at 2-8°C.

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Company: Creative Diagnostics
Phone: 1-631-624-4882
Fax: 1-631-938-8221
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
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