Absolute Mag™ Human CD4 T Cell Isolation Kit

(WCD-K01)

Non CD4+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin nanoparticles. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.


Datasheet

Overview


Application Notes
This kit is designed for the isolation of untouched CD4+ T cells from peripheral blood mononuclear cells (PBMCs).

Kit Components
100 µL of Biotin-Antibody Cocktail: Biotin anti- CD8a,CD11b, CD16, CD14, CD19, CD20, CD36, CD56, TCR-γδ, CD123,CD235ab (Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH7.2.)100 µL magnetic Streptavidin Nanoparticles (Aqueous solution containing BSA and 0.05% sodium azide.)

Isolation Method
Negative Selection

Species
Human

Storage
Antibody cocktail and Streptavidin nanoparticles should be stored undiluted between 2°C and 8°C.

Online Inquiry

Please input "diagnostics" as verification code.
Contact Us
Company: Creative Diagnostics
Phone: 1-631-624-4882
Fax: 1-631-938-8221
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Email:
Copyright © 2009 - Creative Diagnostics. All rights reserved. et: 0.00 ms
Home | Sitemap