Absolute Mag™ Human CD4 T Cell Isolation Kit


Non CD4+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin nanoparticles. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.



Application Notes
This kit is designed for the isolation of untouched CD4+ T cells from peripheral blood mononuclear cells (PBMCs).

Kit Components
100 µL of Biotin-Antibody Cocktail: Biotin anti- CD8a,CD11b, CD16, CD14, CD19, CD20, CD36, CD56, TCR-γδ, CD123,CD235ab (Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH7.2.)100 µL magnetic Streptavidin Nanoparticles (Aqueous solution containing BSA and 0.05% sodium azide.)

Isolation Method
Negative Selection


Antibody cocktail and Streptavidin nanoparticles should be stored undiluted between 2°C and 8°C.

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Company: Creative Diagnostics
Phone: 1-631-624-4882
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